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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential purpose in selling cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been reported but specific nucleotide sequence data is not really nevertheless accessible.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide typically incorporates the epitope recognized because of the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible effects are...
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This information outlines further achievable optimization of cellular and immunoassay parameters to be sure website the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody by yourself, you can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a extraordinary periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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